Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM.

Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM.

Blog Article

BackgroundInfections caused by Klebsiella pneumoniae have been difficult to control because of the worldwide emergence of carbapenem-resistant isolates mainly due to carbapenemase production.Information regarding carbapenemase-producing K.pneumoniae is still scarce in Ethiopia.Therefore, the current study aimed to determine the prevalence of carbapenemase-producing K.pneumoniae and to assess the occurrence of blaNDM and blaKPC carbapenemase genes.

MethodsA cross-sectional study was conducted from September 2018 to February 2019 at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.A total of 132 non-duplicate K.pneumoniae isolates were studied.Phenotypic confirmation of carbapenemase production was done by modified Massage Balls Carbapenem Inactivation Method (mCIM).Multiplex PCR was performed for the detection of carbapenemase-encoding genes blaKPC, and blaNDM.

ResultsOut of the total 132 K.pneumoniae isolates, 39 (29.6%) were non-susceptible to one or more carbapenems.The prevalence of carbapenemase-producing isolates body polish from the total was 28 (21.2%) with mCIM of which the most dominant gene was blaNDM 26 (92.

9%) and one isolate carried blaKPC concomitantly.Carbapenemase-producing K.pneumoniae isolates were 100% non-susceptible to half of the antimicrobials used in the study, including meropenem and ertapenem.Previous use of carbapenems was associated with carbapenemase production (P = 0.004).

ConclusionsThe prevalence of carbapenemase-producing K.pneumoniae isolates was worrying in the study area.To our knowledge, the study described the emergence of blaNDM and blaKPC gene carrying K.pneumoniae in Ethiopia for the first time.Further large-scale molecular-based studies, including other carbapenemase genes and sequencing of K.

pneumoniae, are warranted to have a clear awareness about the presence of antimicrobial resistance high-risk clones in Ethiopia.